What does a CK-MB chemiluminescence analyzer do?

Introduction

The CK-MB chemiluminescence analyzer is a specialized diagnostic tool used to measure the concentration of creatine kinase-MB (CK-MB) isoenzyme in the blood. This enzyme is a crucial biomarker for assessing myocardial damage, including myocardial infarctions (heart attacks). Accurate measurement of CK-MB levels assists healthcare providers in diagnosing and monitoring heart conditions efficiently.

Functionality of CK-MB Chemiluminescence Analyzer

The CK-MB chemiluminescence analyzer functions by detecting specific light emissions generated during a chemical reaction. The process involves binding the CK-MB enzyme to specific antibodies that result in luminescence. The intensity of the emitted light is directly proportional to the concentration of CK-MB in the blood sample. This methodology offers high sensitivity and specificity, making it invaluable in emergency and clinical settings.

Key Parameters and Numerical Analysis

• Detection Range: Typically, modern analyzers have a detection range of 0.1 to 500 ng/mL.
• Assay Time: The time taken is approximately 18 to 30 minutes per sample, facilitating rapid decision-making in critical scenarios.
• Sensitivity: The sensitivity can be as low as 0.1 ng/mL, allowing for detection of even minute changes in CK-MB levels.
• Sample Volume: The required sample volume ranges from 50 to 150 µL, minimizing patient discomfort.
• Reproducibility: Variation coefficient (CV) generally below 5%, ensuring consistent and reliable results.

Company Solutions

Several companies offer CK-MB chemiluminescence analyzers, each with unique features:

1. Roche Diagnostics: The Elecsys CK-MB Immunoassay offers high precision with a detection range of 1.0 – 300 ng/mL and a turnaround time of 9 minutes.
2. Siemens Healthineers: Their Atellica IM CK-MB assay provides accurate measurements with enhanced analytical sensitivity and a broad measuring range up to 500 ng/mL.
3. Beckman Coulter: The Access CK-MB assay is designed for compatibility with the UniCel DxI Immunoassay System and offers a fast throughput with results in as little as 16 minutes.
4. Abbott Laboratories: The ARCHITECT CK-MB assay utilizes chemiluminescent microparticle immunoassay technology and provides reliable detection with a measuring interval of 0.3 – 250 ng/mL.

Clinical Applications

CK-MB chemiluminescence analyzers are primarily used in cardiology departments to:

• Diagnose acute myocardial infarction (AMI).
• Monitor patients with unstable angina.
• Assess patients with suspected cardiac injury.
• Evaluate recurrent ischemia or infarction.

Conclusion

The CK-MB chemiluminescence analyzer is a critical device in modern healthcare. With its precise and rapid measurement capabilities, it plays an essential role in the timely diagnosis and monitoring of cardiac conditions, thereby enhancing patient care and outcomes.

References

1. Roche Diagnostics. (2021). Elecsys CK-MB Immunoassay. Available at: [Roche Diagnostics Website]
2. Siemens Healthineers. (2022). Atellica IM CK-MB Assay Instructions. Available at: [Siemens Healthineers Website]
3. Beckman Coulter. (2021). Access CK-MB Instructions for Use. Available at: [Beckman Coulter Website]
4. Abbott Laboratories. (2023). ARCHITECT CK-MB Assay Datasheet. Available at: [Abbott Labs Website]

Table of Contents

1. Introduction
2. Functionality of CK-MB Chemiluminescence Analyzer
3. Key Parameters and Numerical Analysis
4. Company Solutions
5. Clinical Applications
6. Conclusion
7. References